“Photo-unclick chemistry” and its application in light-controlled drug delivery/release


Spatio-temporally controlled delivery (release) of biologically active compounds is the key strategy to minimize off-target effects of the compounds. For example, chemotherapeutic agents kill not only cancer cells but also certain normal cells causing systemic side effects. To minimize the side effects, the release of anticancer drugs in tumor should be timely controlled. Recently, nano-technology-based drug delivery systems have been developed for site-specific drug delivery of anticancer drugs. While various targeting strategies for site-specific delivery to tumors have been developed, effective releasing methods remain largely un-developed. Visible and near IR light is a very attractive external tool to control the release of drugs, which can reach to deep tissue and does not cause direct photodamage to normal tissue unlike UV. However, the energy of such light is not high enough to directly cleave chemical bonds to initiate the release of drugs. We developed an innovative strategy “photo-unclick chemistry”, by which drugs can be released by visible/near IR light through singlet oxygen. We successfully demonstrated its application in prodrugs. Currently, its application in nano-technology is under way.
  1. Thapa, P., Li, M., Karki, R., Bio, M., Rajaputra, P., Nkepang, G., Woo, S., You, You.* Folate-PEG conjugates of a far-red light-activatable paclitaxel prodrug to improve selectivity towards folate receptor-positive cancer cells. ACS Omega 2017, 2, 6349-6360. [abstract]

  2. Li, M., Thapa, P., Rajaputra, P., Bio, M., Peer, C. J., Figg, W. D., You, Y.,* Woo, S.* Quantitative modeling of the dynamics and intracellular trafficking of far-red light-activatable prodrugs: implications in stimuli-responsive drug delivery system. J. Pharmacokinet. Pharmacodyn. 2017, 44, 521-536. [abstract] Journal Cover Article

  3. Thapa, P., Li, M., Bio, M., Rajaputra, P., Nkepang, G., Sun, Y., Woo, S., You, Y.* Far-red light-activatable prodrug of paclitaxel for the combined effects of photodynamic therapy and site-specific paclitaxel chemotherapy. J. Med. Chem.   2016, 59, 3204–3214. [abstractHighlighted in ChemistryViews

  4. Rajaputra, P., Bio, M., Nkepang, G., Thapa, P., Woo, S., You, Y.* Anticancer drug released from near IR-activated prodrug overcomes spatiotemporal limits of singlet oxygen. Bioorg. Med. Chem. 2016, 24, 1540-1549. [abstract]

  5. Nkepang, G., Bio, M., Rajaputra, P., Awuah, S., You, Y.* Folate receptor-mediated enhanced and specific delivery of far-red light-activatable prodrugs of Combretastatin A-4 to FR-positive tumor, Bioconjugate Chem. 2014, 25, 2175-2188. [abstract]

  6. Bio, M., Rajaputra, P., Nkepang, G., You, Y.*, A far-red light activatable, multi-functional prodrug for fluorescence optical imaging and combinational treatment, J. Med. Chem. 2014, 57, 3401-3409. [abstract]

  7. Nkepang, G., You, Y.*, Heteroatom-substituted dioxetanes and their emerging biomedical applications, Patai's Chemistry of Functional Groups, Edited by I. Marek. John Wiley & Sons, Ltd: Chichester, UK.  Published online 04/28/2014. [abstract] - Invited Book Chapter

  8. Bio, M., You, Y.*, Emerging strategies for controlling drug release by using visible/near IR light, Med. Chem. 2013, 3, 192-198. - Invited Review Article

  9. Bio, M.; Rajaputra, P.; Nkepang, G.; Awuah, S. G.; Hossion, A. M. L.; You, Y.* Site-specific and far-red light-activatable prodrug of combretastain A-4 using photo-unclick chemistry, J. Med. Chem. 2013, 56, 3936-3942. [abstract] - Highlighted in SciBX: Science-Business eXchange

  10. Hossion, A. M. L.; Bio, M.; Nkepang, G.; Awuah, S. G.; You, Y.* Visible Light Controlled Release of Anti-cancer Drug through Double Activation of Prodrug, ACS Med. Chem. Lett.2013, 4, 124-127. [abstract] - Highlighted in the Editor's Choice.

  11. Bio, M.; Nkepang, G.; You, Y.* Click and photo-unclick chemistry of aminoacrylate for visible light-triggered drug   release, Chem. Comm. 2012, 48, 6517-6519. [abstract]

  12. Nkepang, G.; Pogular, P.K.; Bio, M.; You, You.* Synthesis and singlet oxygen reactivity of 1,2-diaryloxyethenes and selected sulfur and nitrogen analogs, Photochem. Photobiol. 2012, 88, 753-759. [abstract]

  13. Murthy, R. M., Bio, M., You, Y.,* Low energy light-triggered oxidative cleavage of olefins, Tetrahedron Lett. 2009, 50, 1041-1044. [abstract]

Combination Therapy for Non-muscle Invasive Bladder Cancers

  1. Bio, M., Rajaputra, P., Lim, I., Thapa, P., Tienabeso, B., Hurst, R. E., You, Y.* Efficient activation of visible light-activatable CA4 prodrug through intermolecular photo-unclick chemistry in mitochondria. Chem. Comm. 2017, 2017, 53, 1884-1887. [abstract]

  2. Bio, M., Rajaputra, P., You, Y.* Photodynamic therapy via FRET following bioorthogonal click reaction in cancer cells. Bioorg. Med. Chem. Lett. 2016, 26, 145-148. [abstract]

Near IR chromophores for fluorescence imaging and photodynamic therapy for theranostics


Photodynamic therapy (PDT) is a unique regime destroying disease targets (cancer cells, hamful vascularture, and bacteira) by reactive oxygen species (e.g., singlet oxygen). Excited photosensitizer transfers its energy to oxygen (photonic energy à
 reactive chemical species). One of the key desired properties of ideal photosensitizers is effective absorption of near IR light to damage targets in deep tissue. On the other hand, optical imaging is a very effective diagnostic tool offering high sensitivity, harmless to tissue, real time monitoring, and low cost. Near IR is also required for optical imaging probe for tissue samples. We are developing new near IR chromophore as theranostic agents (fluorescence detection and PDT treatment). Because fluorescence emission and intersystem crossing (to triplet state for singlet oxygen generation) are competing process, the conventional wisdom was that one chromophore cannot be used for the dual functions. Our strategy is to maximize absorption efficiency (extinction coefficient) with balanced decay for fluorescence emission and ISC. 
  1. Watley, R. L., Awuah, S. G., Bio, M., Cantu, R., Gobeze, H. H., Nesterov, V. N., D'Souza, F.,* You, Y.* Dual functioning thieno-pyrrole fused BODIPY dyes for NIR optical imaging and photodynamic therapy – singlet oxygen generation without heavy halogen atom assistance, Chem. Asian J. 2015, 10, 1335-1343. [abstract]

  2. Awuah, S. G.; Das, S.; D'Souza, F.; You, Y.* Thieno-pyrrole fused BODIPY intermediate as platform for multi-functiional NIR agents, Chemistry - An Asian Journal 2013, 8, 3123-3132. [abstract]

  3. Awuah, S. G.; You, Y.* Boron Dipyrromethene (BODIPY) Based Photosensitizers for Photodynamic Therapy. RSC Advances 2012, 2(30), 11169-11183.[abstract] - Review Article

  4. Awuah, S. G.; Polreis, J.; Biradar, V.; You, Y.,* Singlet oxygen generation by novel NIR BODIPY dyes, Org. Lett. 2011, 13, 3884-3887.[abstract]

Anticancer drug discovery

Through collaborative efforts with biochemists and pharmacologists, we are also trying to discover new anticancer agents with novel mechanims. Conventional medical chemistry approaches are being used: pharomacophore determination, QSAR analysis, and structure optimization.
  1. Chintharlapalli, S., Papineni, S., Liu, S., Jutooru, I., Chadalapaka, G., Cho, S., Murthy, R., You, Y., Safe, S., 2-Cyano-lup-1-en-3-oxo-20-oic acid, a cyano derivative of betulinic acid activates peroxisome proliferator-activated receptor g in colon and pancreatic cancer cells. Carcinogenesis 2007, 28, 2337-2346. [abstract]